Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats

Gamma oryzanol (GO) is well known for its antioxidant activity and health-promoting benefits. The gamma oryzanol-loaded zein nanoparticles (GOZNs) were successfully prepared in our previous study. In the present work, acute oral toxicity of GOZNs was evaluated based on OECD guideline 420. fabricated by liquid-liquid dispersion lyophilized. samples displayed a mean diameter 311.20±3.01 nm high loading capacity 311.22±7.97 to 322.69±5.67 mg-GO/g-powder after 42-day storage at -18 o C. Healthy female Sprague Dawley rats (8 weeks age) used experiments. Five administered single dose 2,000 mg GO/kg body weight via route. Observations signs recorded first 24 hours, changes general physical conditions monitored 14 days before gross necropsy day 15. results show that exhibited normal growth, neither mortality nor observed throughout study period. findings revealed did not have safe when orally short periods with LD50 > mg/kg.